Composite

Part:BBa_M36938:Design

Designed by: Emily Kelly   Group: Stanford BIOE44 - S11   (2014-10-23)


Enoyl-[acyl-carrier-protien] reductase actuator with GFP reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 210
    Illegal XbaI site found at 97
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 210
    Illegal NotI site found at 1006
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 210
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 210
    Illegal XbaI site found at 97
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 210
    Illegal XbaI site found at 97
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1020


Design Notes

No BsaI, BbsI/BsmBI sites. No palindromes >= 10bp.


Source

Composite.

References